@@ -324,25 +324,25 @@ USAGE(S):
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rAMPage.sh [-a <address>] [-b] [-c <taxonomic class>] [-d] [-f] [-h] [-m] [-n <species name>] [-o <output directory>] [-p] [-r <FASTA.gz>] [-s] [-t <int>] [-v] <input reads TXT file>
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OPTIONS:
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- -a <address> email address for alerts
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- -b benchmark (uses /usr/bin/time -pv)
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- -c <class> taxonomic class of the dataset (default = top-level directory in $outdir)
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- -d debug mode of Makefile
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- -f force characterization even if no AMPs found
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- -h show help menu
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- -m <target > Makefile target (default = exonerate )
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- -n <species > taxonomic species or name of the dataset (default = second-level directory in $outdir )
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- -o <directory> output directory (default = directory of input reads TXT file)
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- -p run processes in parallel
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- -r <FASTA.gz> reference transcriptome (accepted multiple times, *.fna.gz *.fsa_nt.gz )
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- -s strand-specific library construction (default = false )
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- -t <int> number of threads (default = 48)
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- -v print version number
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- -E <e-value > E-value threshold for homology search (default = 1e-5 )
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- -S <0 to 1> AMPlify score threshold for amphibian AMPs (default = 0.90 )
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- -L <int> Length threshold for AMPs (default = 30 )
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- -C <int> Charge threshold for AMPs (default = 2)
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-
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+ -a <address> email address for alerts
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+ -c <class> taxonomic class of the dataset (default = top-level directory in $outdir)
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+ -d debug mode of Makefile
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+ -f force characterization even if no AMPs found
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+ -h show help menu
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+ -m <target> Makefile target (default = exonerate)
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+ -n <species > taxonomic species or name of the dataset (default = second-level directory in $outdir )
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+ -o <directory > output directory (default = directory of input reads TXT file )
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+ -p run processes in parallel
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+ -r <FASTA.gz> reference transcriptome (accepted multiple times, *.fna.gz *.fsa_nt.gz)
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+ -s strand-specific library construction (default = false )
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+ -t <int> number of threads (default = 48 )
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+ -v print version number
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+ -E <e-value> E-value threshold for homology search (default = 1e-5)
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+ -S <0 to 1 > AMPlify score threshold for amphibian AMPs (default = 0.90 )
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+ -L <int> Length threshold for AMPs (default = 30 )
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+ -C <int> Charge threshold for AMPs (default = 2 )
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+ -R Disable redundancy removal during transcript assembly
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+
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EXAMPLE(S):
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rAMPage.sh -a user@example.com -c class -n species -p -s -t 8 -o /path/to/output/directory -r /path/to/reference.fna.gz -r /path/to/reference.fsa_nt.gz /path/to/input.txt
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